Journal article
Telomere length varies by DNA extraction method: Implications for epidemiologic research
JM Cunningham, RA Johnson, K Litzelman, HG Skinner, S Seo, CD Engelman, RJ Vanderboom, GW Kimmel, RE Gangnon, DL Riegert-Johnson, JA Baron, JD Potter, R Haile, DD Buchanan, MA Jenkins, DN Rider, SN Thibodeau, GM Petersen, LA Boardman
Cancer Epidemiology Biomarkers and Prevention | AMER ASSOC CANCER RESEARCH | Published : 2013
Abstract
Background: Both shorter and longer telomeres in peripheral blood leukocyte (PBL) DNA have been associated with cancer risk. However, associations remain inconsistent across studies of the same cancer type. This study compares DNA preparation methods to determine telomere length from patients with colorectal cancer. Methods: We examined PBL relative telomere length (RTL) measured by quantitative PCR (qPCR) in 1,033 patients with colorectal cancer and 2,952 healthy controls. DNA was extracted with phenol/chloroform, PureGene, or QIAamp. Results: We observed differences in RTL depending on DNA extraction method (P < 0.001). Phenol/chloroform-extracted DNA had a mean RTL (T/S ratio) of 0.78 (ra..
View full abstractRelated Projects (1)
Grants
Awarded by Lustgarten Foundation for Pancreatic Cancer Research
Funding Acknowledgements
The study was supported by RO-1 CA132718 through the National Cancer Institute (NCI; to L.A. Boardman, J.M. Cunningham, R.A. Johnson, K. Litzelman, H.G. Skinner, S. Seo, C.D. Engelman, D.N. Rider, S.N. Thibodeau, and G.M. Petersen); L.A. Boardman is supported by P30 DK084567 (Mayo Clinic Center for Cell Signaling in Gastroenterology) through National Institutes of Diabetes, Digestive and Kidney Diseases (NIDDK); G.M. Petersen has been awarded an NCI P50 CA102701 (Mayo Clinic SPORE in Pancreatic Cancer) and the Lustgarten Foundation for Pancreatic Cancer Research; the Mayo Clinic Center for Individualized Medicine and by the NCI, NIH under RFA # CA-96-011 and through cooperative agreements with the Australasian Colorectal Cancer Family Registry (U01 CA097735); Familial Colorectal Neoplasia Collaborative Group (U01 CA074799; USC); Mayo Clinic Cooperative Family Registry for Colon Cancer Studies (U01 CA074800); Ontario Registry for Studies of Familial Colorectal Cancer (U01 CA074783); Seattle Colorectal Cancer Family Registry (U01 CA074794); and University of Hawaii Colorectal Cancer Family Registry (U01 CA074806).